Hashimoto’s thyroiditis is a chronic autoimmune thyroid disease characterized by presence of antibodies specific to thyroid antigen especially against thyroperoxidase (anti-TPO), antithyroglobulin (anti-Tg). Homocysteine is sulfur containing amino acid which is produced during catabolism of essential amino acid methionine. Autoimmune thyroiditis is often associated with autoimmune gastritis which has parietal cell antibodies (PCA) in the circulation and it directs against the proton pump (H+/K+ ATPase) and intrinsic factor resulting into atrophic gastritis . This atrophic gastritis reduces the availability of functional gastric mucosa and the ability to secrete intrinsic factor which causes malabsorption of vitamin B12 and so elevated level of homocysteine level. Hyperhomocysteinemia is connected with coronary heart disease (CHD) and cardiovascular diseases (CVD) by endothelial dysfunction and induces apoptotic cell death. Hence this study can help in early diagnosis of cardiovascular diseases in Hashimoto’s thyroiditis. Objective : Aim of the study is to evaluate the serum total homocysteine level and serum TSH level in patients with Hashimoto’s thyroiditis and also to find if there is correlation between TSH level and homocysteine level. Material and Method : A total 84 individuals were participated into the study : one group had 42 diagnosed patients of Hashimoto’s thyroiditis of age group 20-55 and another group had 42 age, sex matched healthy controls. Serum total homocysteine level and serum TSH level were assayed by Enzymatic method and electrochemiluminescence method respectively. Result : Mean age (35 ± 9.71 Vs. 38 ± 7.99 years ,p=0.13). Thirty three (78.5%) cases and 30 (71.4%) control were females. Serum TSH level (6.49 ±1.17 Vs. 1.5 ± 0.71 mIU/L, p<0.0001) which was significantly higher in cases as compared to control. Serum total homocysteine level (18.0 ± 3.66 Vs. 8.2 ±2.61 μmol/l, p<0.0001) was significantly higher in patients as compared to control. Positive correlation was found between TSH level and total homocysteine level (r=0.96). Conclusion : Positive correlation between TSH level and Homocysteine level in Hashimoto’s thyroiditis contribute to increased cardiovascular risk and we can consider total homocysteine level as screening in Hashimoto’s thyroiditis