This research paper attempts to scrutinise the essence of depiction of ‘Biological Disease HIV+ AIDS’ mirrored in a sample Indian Hindi LGBTQ+ motion picture. The sample film selected for this research is the Indian Hindi language Gay moving image My Brother Nikhil (2005) directed by Onir. The qualitative data have been collected through the application of content analysis and critical review technique followed by the analysis of the data. Finally, it has been discovered from this investigation that the cinema My Brother Nikhil exhibits the psycho-biological and socio-cultural taboo and sufferings caused by HIV+ AIDS Biological Disease. The protagonist of the film Nikhil has been forcefully quarantined in a dirty room having violated universal human rights by Goa police for being HIV positive, from which he is rescued by his elder sister and boy friend having encountered legal battle in court. The findings of this post-mortem contribute to the arena of Biological Sciences in general as well as Medical Sciences, Nursing, Pharmacology, Biochemistry, Biotechnology, Zoology, Immunology, HIV+ AIDS, Gender Studies, Queer & Sexuality Studies, Gay Studies, Sociology, Law and Juridical Studies, Human Rights, Film Studies, Journalism & Mass Communication and several other homologous disciplines in specific.