Aim of study: The objective of this study was to optimize the germination traits (germination rate, Germination kinetics and Radicle length kinetics ) of Moringa Oleifera seeds after different pre-germination treatments such soaking seeds for 24, 48, 72 and 96 hours and physico-chemical treatments of soaking the seeds in oxygenated water for 10min seed coat removal. Area of study: The study was conducted in the laboratory on M. Oleifera Lam. cultivar originally from Tebelbella (400 km south of Bechar, Algeria). Material and methods: Laboratory experiment was designed to investigate the influence of different pre-germination treatments such soaking and physico-chemical treatments on Moringa Oleifera germination traits. Main results: Seed coat removal treatments gave the highest values on germination rate after 13 days of germination. Prolonged soaking treatment has a negative effect on seed germination, leading to a significant reduction in germination rates. Germination kinetics enhanced by both of chemical (H₂O₂) and of physical (seed coat removal) treatments. Seed coat removal exhibited the highest values for root elongation among all the treatments