Introduction: Heart Failure (HF) associated with significant morbidity and mortality. Normally, circulating levels of BNP and NT-proBNP are quite low, but the levels are increased in HF. This study aimed at: (1) To assess the role of NT-proBNP in diagnosis of HF. (2) To study the effect of various factors like age, BMI on NT-proBNP. (3) To find a relation between NT-proBNP levels and left ventricular ejection fraction (LVEF), disease severity and aetiology of HF. Materials and methods: In this study, 100 patients of both genders having cardinal signs of HF were included. Blood samples were collected and used for estimation of NT-proBNP, urea, creatinine, RBS using autoanalyzer. Electrocardiogram, chest X-ray, and 2D Echo were obtained. 2D-Echo was used to confirm the diagnosis of HF and for estimation of LVEF.