This study focuses on the comprehensive analysis of Paeonia emodi extract, including its phytochemical composition and pharmacological potential. Various solvents were used to extract bioactive compounds from Paeonia emodi, and their yields were determined. Phytochemical screening revealed the presence of glycosides, flavonoids, phenols, proteins, carbohydrates, and saponins in the extract. Quantitative estimation showed significant levels of total phenols and flavonoids, particularly in the ethanol extract. The extract exhibited notable antioxidant activity, as evidenced by its inhibition of DPPH and hydrogen peroxide radicals. Furthermore, quercetin, a potent antioxidant flavonoid, was quantitatively determined in the extract. These findings suggest that Paeonia emodi extract possesses promising antioxidant activity, which may have therapeutic implications in various health conditions.