Polymorphisms that increase risk factor for male infertility is one of the most studied infertility genetic factor however polymorphisms within premiRNA sequences have not extensively documented. This study aimed to highlighted association between abuse anabolic steroids and male infertility by evaluate the genotyping and allele frequency of androgen receptor gene of rs1337082, and found the relationship of this SNP and some serum hormones levelsThe samples for this study were obtained from the Kamal Al- Samara'ay IVF Hospital, Ministry of Health in Baghdad, Iraq.