Semicarbazide derivatives have garnered a lot of interest because of their wide variety of biological effects and function as building blocks in numerous families of important pharmacological molecules. Type 1 diabetes usually affects kids or teenagers, while type 2 diabetes mellitus usually affects middle-aged and older adults for long period of time due to poor food and lifestyle choices. The present research was based on the synthesis and evaluation of in-vitro anti-diabetic potential of novel derivatives of semicarbazide.To design, synthesize the novel derivatives of semicarbazide. The novel derivatives of semicarbazide were synthesized and characterized based on physicochemical properties i.e., melting point, Rf value, FTIR, NMR and Mass spectroscopy methods. They were also evaluated for the in-vitro anti-diabetic potential of synthesized novel derivatives i.e., estimation of DPPH Radical Scavenging Assay and Estimation of α-amylase. In results, the Rf values for C1 to C6 were found to be 0.65, 0.67, 0.71, 0.69, 0.66, and 0.72, respectively. The α-Amylase inhibition is an important feature for estimation of anti-diabetic potential. At 800μg/ml, the α-Amylase inhibition was estimated as 45.34±0.11%, 63.45±0.21%, and 91.35±0.46%, in water, semi-carbazide derivatives and acarbose, respectively. In conclusion, all the synthesized semi-carbazide derivatives demonstrated s significant antioxidant activity and α-amylase inhibition when compared with control. On the basis of physicochemical parameters i.e., physical state, melting point, FTIR, NMR, mass studies, the derivatives were confirmed for the novel derivatives of semi-carbazides. Further research may carried-out to confirm its mode of action and optimum doses required in hypoglycemic action.