2-amino-3-cyanopyridines were prepared by using 4-hydroxy acetophenone as starting material which is treated with malononitrile and various types of benzaldehydes consists of electron withdrawing groups on it. Solvent used in this reaction condition is toluene, which gives more yield when compared with using of other solvent, the synthesized 2-amino-3-cyanopyridines were characterized by physical properties and spectral studies (IR, 1H NMR). 5-substituted-7-(4- hydroxyphenyl)-pyrido [2,3-d] pyrimidines were synthesized by taking 2-amino-3-cyanopyridines as starting materials, which were treated with PhNCS/Urea/Thiourea. The synthesized 5-substituted 7-(4-hydroxyphenyl)-pyrido [2,3-d] pyrimidines were screened for antibacterial and antimitotic activity. Among all the synthesized compounds Compound 5C showed significant activity against Bacillus subtilis. From the results of antimitotic activity, all the title compounds (5a-7c) showed dose dependent inhibitory effect on seed germination, radical length, and mean weight of seedlings. Among all these 6b, 6c and 7b shows significant results on decrease percentage germination and radical length gained, mean weight at 2.5 mg/mL and 5.0 mg/mL as par with aspirin 2.5 mg/ml.