This study uses in vitro evaluations to examine the nephroprotective and antidiabetic properties of a herbal formula containing methanol leaf extract of Balbisia pedunculata and Allium sativum (HF-ASBP). An examination of the herbal formula (HF-ASBP) revealed information on how it affected the activity of DPP-IV and the uptake of glucose by L6 myoblast cells. Although there was a slight drop in blood glucose levels and an increase in glucose absorption in L6 cells, HF-ASBP did have a small antidiabetic impact. However, as there was no significant DPP-IV inhibition, further research is required to determine the precise biochemical mechanisms at play. Concurrently, the nephroprotective capacity of HF-ASBP is examined in relation to cytotoxicity generated by cisplatin in HEK-293 cells. Surprisingly, HF-ASBP showed a strong cytoprotective effect, improving cell survival linked to kidney damage caused by cisplatin. These results establish HF-ASBP as a potentially useful natural herbal formula for nephroprotective treatment by reducing drug-induced kidney damage. As a versatile botanical option for nephroprotective therapies and diabetic management, HF-ASBP exhibits potential. Further research into the particular bioactive chemicals in HF-ASBP, their mechanisms of action, and their potential benefits to reduce the global burden of diabetes and renal disorders is required in light of the complex findings observed in vitro.