Background and Objectives: Zinc (Zn) is an important trace element with substantial consequences for health, and its blood levels might fluctuate depending on demographic variables. This study aimed to explore the relationship between serum Zn levels and age and sex in the population ofErbil City, Iraq. Methods: This cross-sectional study encompassing 844 participants that had referred to Mydia Diagnostic Center from January 2021 to December 2022. Participants were evaluated at baseline and followed up at a specified interval (24 months). Serum Zn concentration was measured using atomic absorption spectrophotometry. Results:The mean age of participants was 39.789 ± 20.427 years. The mean serum Zn concentration among all participants was 80.678 ± 16.893 μg/dl. The results indicated that despite fluctuations in Zn levels and an overall increase among participants, Zn deficiency remains prevalent. It was observed that Zn levels decreased with age, and females exhibited lower Zn levels than males across different age groups.