Introduction. In the modern world, cervical cancer is one of the most common cancers of the female genital area and occupies one of the leading places in the structure of mortality. The purpose of this study was to determine the significance and value of biochemical and immunocytochemical (IC) diagnostic markers for HPV in women with background and precancerous diseases in the cervix. Materials and Methods: Examined 152 women with precancerous and background diseases of the cervix (erosions and endocervicitis), whose age ranged from 23 to 59 years, with an average of 33 ±1,9 years. All 152 patients with underlying conditions of cervical cancer underwent Pap smears, liquid cytology, PCR test to identify the HPV virus, and verification of specific proteins p16/ki67. Research results. All patients underwent a cytological examination of the cervix using the Pap test and liquid cytology with interpretation of the results according to the Betesta system (2014). This means that in comparing two methods for identifying intraepithelial lesions of the cervix in two groups, false-positive results were in 11 and 10, and false (+) results were in 9 and 3 in the two corresponding groups. And 6 in 2-group. According to WHO recommendations, for verification, NSIL requires intensive treatment; using a surgical method, samples with ASCUS and LSIL should be subject to strict monitoring, since in the future they may develop into severe dysplasia. Conclusion. In patients with CIN associated with HPV, persistent PVI was present in 83.3% of cases. CIN II-III showed significant overexpression of biomarkers p16ink4α and Ki-67, unlike CIN I. New diagnostic technologies promise advancements in cervical screening and treatment.