The objective of this study is to investigate the protective effect of Negella Sativa (N.sativa) seedsagainstthetoxicity ofreproductivemarkersinadult malerabbitstreated withaluminum chloride(AlCl3). Sixteenrabbitswererandomlyandequally divided into fourgroups,thefirst grouptaken as control (T),the second group(NS) wastreated with200 mg/kg/day of Nigella Sativa seeds extract; the third group (AL) was treated with 25 mg/kg/day of AlCl3, and The last group (NS-AL) received the combined treatment of AlCl3 and N. sativa extract at the same doses.The administration of AlCl3 and seed extract was carried out orally for four weeks. The results showthat the Al-treated group (AL) had reduced testicular weight, plasma levels of testosterone, LH, FSH, sperm speed, motility, and concentration compared to the control group (T). Furthermore, a microscopic examination revealed many histological alterations in the interstitial tissue and seminiferous tubules. The reproductive toxicity of AlCl3 resulted in testicular degeneration in the majority of seminiferoustubules,accompanied byadecreasein the number of germinal cell layers in the seminiferous tubules, and the accumulation of multinucleated giant cells in the tubular lumen. In contrast, the groups treated with N.sativaalone (NS) or combined with aluminum (AL-NS) showed that the histopathological alterations were significantly attenuated by the administration of N. sativa extract. Based on theresults ofthis work,it seems thatthe supplementation of N. sativa extract may protectthe reproductive system from damage induced by AlCl3