Allergic rhinitis (AR) is the predominant non-infectious form of rhinitis characterized by symptoms such as sneezing, coughing, and flu-like manifestations. The precise pathophysiology of AR is yet unknown. Vitamin D3 insufficiency has been identified as a likely cause of allergy disorders because of its function in immunomodulation. Aim: To estimate serum Vitamin D3 and inflammatory markers (IgE, IL-6) in patients of allergic rhinitis and healthy controls. Materials and Methods; The present study was conducted in the Departments of Biochemistry and the Department of ENT, SGT Medical College, Hospital & Research Institute, Gurugram, Delhi-NCR, India. A written and informed consent was taken after explaining the purpose and details of the study to all the subjects of both the groups. A total of 160 individuals with clinically confirmed allergic rhinitis, aged between 18 and 55 years, were seen in the ENT outpatient department at SGT Hospital. A total of one hundred and sixty healthy volunteers, matched in terms of age and gender, was selected from the general public to serve as controls at SGT hospital. The measurement of Serum Vitamin D was conducted using a Competitive ELISA kit-based approach, whereas the measurement of Serum Interleukin 6 and IgE was conducted using a Sandwich ELISA kit-based method. Results: It was observed that patients in the AR group had an average blood vitamin D level of 11.32±3.96 ng/ml, whereas participants without AR had a level of 27.15±16.26 ng/ml. The observed differences were found to be statistically significant with a p-value of 0.0001. The control group had a mean serum IgE level of 36.08±19.43 IU/ml, whereas the study group had a mean serum IgE level of 269.19±71.27 IU/ml (p<0.0001). The control group had a lowest value of 4.1 and a maximum value of 82.6, whereas the study group had a minimum value of 167.7 and a maximum value of 500.3. The mean serum IL6 levels in the control group were 6.67±6.55 IU/ml, but in the study group, they were 26.89±22.24 IU/ml (p<0.0001). The control group had a lowest value of 0.9 and a maximum value of 40.5, whereas the study group had a minimum value of 3.9 and a maximum value of 89.7