Liver cirrhosis represents the final stage of various chronic liver diseases, with fibrosis serving as its precursor. Activation of hepatic stellate cells (HSCs) is a crucial event in the development of fibrosis. While synthetic drugs exist to alleviate liver cirrhosis, they often come with adverse effects, notably hepatotoxicity. Paracetamol, a widely used analgesic and antipyretic drug, can induce liver toxicity, particularly at high doses. One of its metabolites, N-acetyl-p-benzoquinone imine (NAPQI), depletes liver glutathione levels and directly damages hepatic cells. This study explores the hepatoprotective potential of Pentas lanceolata against paracetamol-induced liver toxicity. The solvent extract of Pentas lanceolata leaves, belonging to the Rubiaceae family, was obtained through Soxhlet extraction. Phytochemical screening of the plant extract revealed the presence of various metabolites, including alkaloids, carbohydrates, flavonoids, quinines, and phenols. In vitro experiments involved administering the extract to rats, with Silymarin serving as a standard drug for comparison. Biochemical markers such as ALT, AST, ALP, albumin, and total bilirubin were evaluated in the liver cells of the rat model. Histopathological examination confirmed the hepatoprotective activity of Pentas lanceolata against paracetamol-induced toxicity.