Acute bacterial conjunctivitis is a common ocular condition that often requires antibiotic therapy for resolution. Lomefloxacin and ofloxacin are two commonly used topical antibiotics for this indication, but their comparative efficacy remains unclear. Objective: To compare the efficacy of topical lomefloxacin and ofloxacin in managing acute bacterial conjunctivitis. Methods: This was a randomized controlled trial conducted at a Gremers medical Institute. Fifty two subjects were enrolled in the study from September 1992 to July 1994 and were randomly allocated to either lomefloxacin 0.3% eye drops administered twice daily or tobramycin 0.3% eye drops administered four times daily. Evaluation occurred at baseline, on day 3–4, on day 7–8, and for patients with residual inflammation/infection on day 7–8, on day 14–18. Symptom severity was assessed using a sum score of individual key signs and symptoms, including itching, foreign body sensation, ocular pain, dry eye sensation, watering eye, and chemosis, as well as other signs and symptoms such as corneal infiltration, follicles, papillae, and red eyelids. Results: Both lomefloxacin and ofloxacin effectively alleviated key signs and symptoms of acute bacterial conjunctivitis. There were no significant differences between the two treatment groups in terms of improvement in key signs and symptoms, other signs and symptoms, or total signs and symptoms at days 3-4 and 7-8 compared to baseline. Conclusion: Topical lomefloxacin and ofloxacin demonstrated comparable efficacy in managing acute bacterial conjunctivitis. These findings support the use of either antibiotic for the treatment of this common ocular condition.