52 years old male, Balinese, patient came to the hospital due to hematemesis one day prior to admission. In the emergency room, the doctor stabilizing patient condition with prompt treatment and giving intravenous proton pump inhibitor. Upper gastrointestinal endoscopy is done after improving of patient condition. We found a solitary giant ulcus in the bulbous duodenum area. Duodenal biopsy was performed and the Pathologist found rod-shaped microorganisms, confirming Helicobacter pylori (H. pylori) involvement. Polymerase Chain Reaction and sequencing of H. pylori revealed an EPIYA-ABD motif with unique motifs EPIYT on second EPIYA sequence. This is the first time in Bali we have sequencing of H. pylori gene, then isolate of H. pylori named then as H. pylori Bali 03 isolate. Standard protocol of H. pylori eradication is conducted by administration of triple-drug therapy consisting of proton pump inhibitor (PPI), two antibiotics such as clarithromycin and amoxicillin, and for 14 days. Patient discharged from hospital in stable condition with successful treatment of H. pylori. Esophagogastroduodenoscopy reevaluation was done in the next 3 months later and revealed completely ulcer resolved.