Introduction: Respiratory tract infections, especially lower respiratory infection (LRTI) is the most common infection, accounting for over 90% cases, with over 7 million deaths annually. India, being a developing country, accounts for 25% of tuberculosis worldwide. Diagnosing and differentiating Tuberculosis from other LRTI pose a severe challenge. There are many microorganisms which can lead to LRTI, commonest being Streptococcus pneumoniae, Klebsiella species etc. Emergence of antibiotic resistance is a major concern as it leads to significant increase in the mortality and morbidity. Aim: the aim of this study is to identify the bacterial pathogens isolated from sputum samples suspected of tuberculosis. Materials and