Five different localities from Pravara area (Songoan, Kanadgoan, Dadh , Hanumatgoan, Tambhere) were selected for isolation of R.meliloti strains and labeled as R1, R2 R3 R4& R5.the strains were tested for Varietal Response of Trigonellafoenum-graecum (fenugreek) against Rhizobium meliloti L. strain’s individual and mixed strain combination in this present investigation it was proved that as per the variety and strain combination the growth parameters i.e. percent germination , root length, shoot length, plant height,number of leaves, and biomass showed variation hence there is a urgent need for further investigation on this topic for increasing yield of farmers to boost their economy.