Background:Numerous researches have demonstrated that DM susceptibility is associated with polymorphisms in the VDR gene. But the findings have been conflicting, varying depending on the location. The purpose of this study was to assess the relationship ofvitamin D level and VDR gene polymorphisms with DM in Egyptian patients. Methods: Enrollment consisted of 50 T1DM cases, 50 T2DM cases, and 50 healthy volunteers. By ELISA, 25hydroxyVDlevel was determined. PCR-RFLP was used to detect VDR gene polymorphisms. Results: Compared to healthy individuals, the mean serum VD level was lower in diabetic cases. Regarding the VDR BsmI genotype, there was no statistically significant variation seen between the case and healthy individuals. Regarding the FokI genotype, there was a significant variation between the T2DM and healthy individuals. The FF genotype had a greater risk of T2DM than the Ff genotype (odds ratio: 12.80). The distribution of the Fok-I genotype showed a significant variation between the T1DM and T2DM cohorts; 8% of TIDM had the ff genotype, compared to 0% in T2DM (P=0.039). Conclusion:The relationship between T2DM in the Egyptian population and the VDR gene FokI polymorphism is supported by this study. And there's no connection between DM and BsmI polymorphism.