The study involved the extraction of 9 NAFLD samples from the NAFLD single-cell dataset GSE136103, which was obtained from the GEO database (www.ncbi.nlm.nih.gov/geo). The data were analyzed using the "Seurat" package in R software version 4.0.5 (FIG 1A-B). The annotated macrophage population was compared to other populations (FIG 1C) simultaneously, and a set of differentially highly expressed genes in macrophages was obtained (Table 1). The NAFLD dataset GSE61260 was downloaded from the GEO database (www.ncbi.nlm.nih.gov/geo), in which the expression matrices of the whole genome of 47 NAFLD and 62 normal control samples were downloaded, and utilized the "limma" package of the R software(version 4.0.5) to " package for differential analysis of the above data (FIG 1D-E). The single-cell dataset GSE136103 was used to obtain the macrophage differentially highly expressed gene set and the GSE61260 differentially highly expressed gene set was intersected to obtain the key gene LYZ (FIG 1F).