Background: Home accidents, characterized by their unexpected and unplanned nature, often result in fatal or severe outcomes. Common types of home accidents include burns, falls, drowning, poisoning, suffocation, and injuries caused by sharp objects. Aim: The study aimed to assess the Knowledge regarding Prevention of Home Accidents among mothers of under five children in selected hospital, Chennai. Methods: Descriptive Cross section design was used for the study. The study was conducted among 80 mothers of under-five children in a selected hospital at Chennai. The samples were selected using a convenient sampling technique who fulfils inclusion criteria. Finding: The study revealed that the majority (52.5%) of the respondents had moderate knowledge, a small percentage (3.8%) had adequate knowledge, and 43.8% had inadequate knowledge on preventing home accidents. Conclusion: The study concluded revealed that the majority of the mothers had moderate knowledge. The analysis found no significant association between knowledge levels and selected baseline variables such as age, religion, family type, number of children, mother's education, or child accident history.