This study aimed to evaluate the phytochemical components and biological properties of two Centranthus ruber extracts. The total polyphenols and flavonoids content were evaluated, and the highest total phenolic content was determined in ethyl acetate extract (337.7±7.8 μg EAG/mg). Significantly highest total flavonoid content value for the nbutanol extract at 63.7±3. 8μg EQ/mg. The n-butanol extract was analyzed by HPLC, and sixteen compounds were determined including flavonoids and phenolic acids. The results also show that n-butanol extract contains Chlorogenic acid (646.73 mg/kg) as a major concentration, followed by Hesperidin (341,09 mg/kg) and P-Coumaric acid (101.45 mg/kg). Using different in vitro assays including DPPH, FRAP methods, and β-carotene bleaching assay, the extracts exhibited significant antioxidant activity. The n-butanol extract showed higher activity of antioxidant assays than the ethyl acetate extract. The disk diffusion method was used to evaluate the antibacterial activity of the extracts against three microbial strains. The n-butanol extract exhibited higher zone inhibition 13±0.1, 17.1±0.05 and 15±0.13, respectively against the tested bacterial strains Pseudomonas aeruginosa, Bacillus cereus and Staphylococcus aureus.