Background: Play is essential for children's development for numerous reasons. Play encourages creativity and the development of imagination, dexterity, and physical, cognitive, and emotional strength. Aim: The aim of the study to assess the knowledge regarding the importance of play therapy for hospitalized children among Mothers in selected hospital. Methods: Descriptive research design was used. The study was conducted among mother with children who were admitted in a selected hospital at Chennai. The sample consisted of 84 mothers with children. A convenient sampling technique was used for the study. Finding: The study revealed that in terms of knowledge about play therapy, 75% demonstrated a satisfactory understanding of the introduction, and 60% understood its definition. However, only 55% were aware of the types of play, and just 47.85% understood the advantages of play, indicating significant gaps. Knowledge of safety precautions was relatively higher at 57%. Conclusion: The study concluded revealed that mothers had moderate knowledge on play therapy. They need the educational programs to enhance mothers' understanding of play therapy's benefits and types. No significant association was found between demographic variables and mothers' knowledge of play therapy (p>0.05).