Wild onions, members of the Allium genus, have received attention for their high concentration of bioactive chemicals, including allantoin, which is known for its medicinal qualities. This research focuses on enhancing the extraction technique for allantoin from wild onions, as well as defining its yield and purity levels. Fresh wild onions were picked, cleaned, and extracted using a variety of techniques, including solvent extraction, ultrasound-assisted extraction, and enzymatic hydrolysis, under varying circumstances. The extracted allantoin was measured with high-performance liquid chromatography (HPLC) and evaluated for purity and possible uses. To increase the yield of allantoin, optimization factors such as solvent type, extraction temperature, duration, and solvent-to-sample ratio were carefully assessed. When compared to other procedures, solvent extraction using ethanol at high temperatures produced the greatest yield of allantoin. Furthermore, HPLC analysis showed allantoin as the primary bioactive ingredient in the extracts, with high purity levels. This work sheds light on the extraction of allantoin from wild onions, with possible uses in medicines, cosmetics, and dermatological goods. The optimized extraction methodology given herein serves as a foundation for obtaining the therapeutic advantages of allantoin from natural sources, hence contributing to the development of sustainable and environmentally friendly alternatives in a variety of sectors.