The goal of the current study was to evaluate the suitability of several diets for Heteroneustes fossilis in a laboratory setting. Heteropneustes fossilis were collected from Sarua Lake located at Gorakhpur for this study and kept for two days in a big rectangular aquarium for acclimatization. Thirty fish were used in the feeding study, which lasted 30 days. The fish were kept in cement aquaria of 54×36×36 cm3, each holding 30 L of water, and their minimum and maximum lengths were 92.7 and 101.2 ± 2.3 mm and their weights were 3.2 and 5.7 ± 0.45 gm respectively. Three separate feeding trials were conducted: one for tubifex, one for commercial pellet feed, and one for a mixed diet consisting of food prepared with dried fish flour, dried prawn flour, wheat flour, and mustard oil cake, along with a vitamin B complex. The fish were given food twice a day until they were satisfied. In comparison to other diets, the fish fed a mixed diet along with tubifex diet had considerably better outcomes in terms of length. Throughout the trial periods, water quality measurements were tracked and through this study we found that Heteropneustes fossilis fish benefit from a mixed diet without tubifex diet.