Background: Sugarcane (Saccharum officinarum L.) is an agricultural commodity that plays a role in the production of granulated sugar, has economic value, and is a source of state income. There is a decline in sugarcane productivity due to the low number of superior sugarcane seeds. One way is to provide molecular markers for kinship studies. Kinship can be identified based on the similarity of characters due to genetic composition. Objective: This research aimed to analyze the quality and quantity of total DNA in commercial varieties at PT Gunung Madu Plantations (GMP). Methods: This research was conducted at the PCR Laboratory in June-August 2023. The plant material used was leaves from 12 commercial varieties that were 29 days old. Sugarcane leaf samples were isolated and extracted using the CTAB method. The DNA quality test used 1% agarose and the quantity test was carried out using a µDrop spectrophotometer at an absorption ratio of 260/280. Result: The research showed that DNA fragments had the thickest and brightest color in samples 2, 6, 8, 9, and 13. The DNA purity of the samples ranged from 1.80-1.93 with a concentration between 394-1400 µg/ µL. The lowest DNA concentration was in the GM 869 and the highest was in the GM 1834 variety. Both of DNA quantity and quality show that GM 1686, GM 1834, and GM 108 have high concentrations with thick and bright bands Conclusion: From the above findings, it can be concluded that GM 1686, GM 1834, and GM 108 varieties have high purity (1.87-1.93) and concentrations (1,332-1,429ng/ μL) with thick and bright bands.