Background: Gallstone disease is common worldwide, affecting 10-20% of the population and causing serious complications such as biliary colic and obstructive jaundice. Understanding anatomical variances in the biliary system, particularly structures such as Calot's triangle, is critical for performing safe surgical treatments. Aim: The purpose of this observational study was to look into anatomical changes in the extrahepatic biliary system using embalmed human cadavers, specifically the union of the cystic duct with the common hepatic duct, duct lengths, and variations in Calot's triangle.