The microbial diversity and ecological dynamics of extreme environments such as Sambhar Lake, Rajasthan, are of significant interest due to their unique characteristics and potential biotechnological applications. In this study, Physicochemical analysis is done from various sitesin Jaipur district. Different parametersincludecolour, temperature, pH, Total solid suspension (TSS), total dissolved solids (TDS), Turbidity, Total alkalinity, Total hardness, calcium,and presence of chloride by prescribed methods.The results highlight the presence ofthe extreme conditions of Sambhar Lake and marble slurry site, contributing to the overall microbial diversity of the ecosystem. The insights gained from this assessment contribute to our knowledge of physical-chemical differences in extreme environments and hold potential implications for biotechnological applications, environmental monitoring, and conservation efforts in saline ecosystems.