This study explores the phytochemical composition and therapeutic potential of Bunium mauritanicum tubers, with a focus on their Antimicrobial and proteinase K inhibitors properties. Quantitative analysis confirmed higher polyphenol and flavonoid contents in methanolic extract (0.509 ± 0.141 mg of GAEq/g of extract and 0.097 ± 0.030 mg QEq/g of extract) compared to aqueous extract (0.352 ± 0.112 mg of GAEq/g of extract and 0.078 ± 0.023 mg QEq/g of extract).In antimicrobial activity, methanolic extract demonstrated bactericidal effects against E. coli, K. pneumoniae, and S. aureus, with MBC/MIC ratios < 4. However, it displayed moderate activity against Candida albicans (MBC/MIC ratio= 1). The proteinase K inhibitor assay showed a significant decrease in enzymatic activity, potentially reducing the risk of heart disease, stroke, and cancer.This study highlights the multifaceted therapeutic potential of B. mauritanicum tubers as a source of bioactive compounds with promising health implications.