Background: Ventilator-associated pneumonia (VAP) is the most common nosocomial infection in people receiving mechanical ventilation. This study aims to detect the etiological agents of VAP and determine their antibiotic susceptibility pattern. Methodology: This cross-sectional study was conducted among patients admitted to the intensive care unit at a single tertiary care center in south India. All patients who developed VAP in the ICU during the study and qualified for the inclusion criteria were investigated clinically, radiologically, and on a microbiological basis. Clinical history and examination- Relevant clinical history with patient symptoms was noted. Patients suffering from pneumonia on admission or during the first 48 hours of mechanical ventilation were excluded. Results: The total of 70 patients fulfilling inclusion criteria were included in the present study. The mean age of participants was found to be 49.33±16.46yrs of age, with majority of patients in the age group of 40-60yrs of age. In present study, we have seen male preponderance with 84.3% were male and 15.7% were female patients, with male to female ratio of 6:1. 30% of them had the gram positive infections and 70% had the gram negative infection. Majority were infected with Klebsiella pnemoniae (27.1%), followed with Enterobacter cloacae (14.3%), Acinetobacter baumannii (12.9%), Pseudomonas aeruginosa (10%) and CONS in 8.6%. More than 30% of sensitivity was seen with Tigecycline, followed by more than 20% sensitivity to the gentamycin. Conclusion: The prevalence of multidrug-resistant microorganisms is at an increasing rate. Among them, gram-negative organisms are more predominant than gram-positive organisms. The resistance pattern of these infections can assist a facility in developing an efficient antimicrobial policy