Introduction: Candida nosocomial infection and multidrug resistance have become a major threat in health care setup. Candida, an opportunistic pathogen, colonizes humans at various sites like the skin, mucous membrane of the Gastrointestinal tract, Respiratory system etc. In most cases of Candida infection, isolated species are found to colonize the host. However, reports suggest transmission by hand of those colonized by the opportunistic pathogen and by fomites. Over recent decades, Candida auris has emerged as a pathogen known to cause serious nosocomial infections. The present study was carried out to identify Candida species colonization among various Health care workers (HCWs) in Intensive care units (ICU) and to know if Candida auris is among the Candida species isolated. Materials and Methods: Swabs from both hands, nostrils, and oral cavity were collected from 125 healthcare workers of ICUs, at the Tertiary Health care centre of North Karnataka, between December 2022 and February 2023. The samples were cultured on Sabouraud’s Dextrose agar. Morphological characterization of yeasts was done using gram stain, germ tube formation test, and Hi-Crome Candida differential agar.