As world economy develops, with advance and updated lifestyle profoundly, the chronic diseases happenings rise continually. Cardiovascular diseases (CVD), is group of disorder and leading cause of mortality from last three decades. Despite significant advances in cardiovascular disease in prevention and treatment, the morbidity and mortality increase continually in developed countries. Underlying causes of cardiovascular disease are multiple factors, complex mechanisms and some common components at end stage that carries thrombosis. Number of safe and effective antithrombotic drugs plays critical role in the treatment of cardiovascular diseases. Objective: The Insilco methodologies are in use now-a days can impacts the entire drug development process as identifying and discovering new potential drug is time and cost effective. Here, we are using different Insilco methodologies to study FXa inhibition activity. Methods: In our present work, we screened and assesses the toxic profile of the selected ligands by OSIRIS property explorer and TOXTREE web server online source in order to obtain the novel or potent molecule for designing. Result: In our present work, total seven compounds were taken and using in-silico approach only three L1, L4 and L5 showed a remarkable binding energy, therapeutics activity in addition to pharmacological activity. Conclusion: Further, directional approach is also need in clinical trials and commercialization. All the selected ligands satisfactorily accepted and developing a new agent.