Simvastatin is a widely prescribed drug for management of dyslipidemia. It is reported to have low oral bioavailability possibly due to poor oral absorption or extensive first pass metabolism. In the present study, beneficial effect of bioenhancer piperine on the intestinal model in rats. Abdomen of overnight fasted anaesthetized rats (3 per group) was cut common and the jejunum segment (10 cm) of small intestine was exposed and cannulated at both the ends using PE tubings. Through one end buffer solutions containing either simvastatin (30 µg/mL) or simvastatin (30 µg/mL) in combination with piperine (20 µg/mL) were perfused at a flow rate of 0.2 ml/min and the perfusate samples were collected at different time points up to 2 h. Phenol red (30µg/ml) was added to both the solutions as non-absorbable biomarker to calculate net water flux. The samples were estimated for simvastatin levels and effective permeability (Peff) was calculated. The Peff value estimated for the combination group was significantly improved compared to alone simvastatin group, suggesting beneficial role of piperine in the oral absorption of simvastatin. Thus piperine can be combined with simvastatin to improve its oral bioavailability, however further studies are required to confirm this findings.