Fenugreek (Trigonella foenum-graecum L.) is an annual herb belonging to the Fabaceae family. It is found all over the world, but it is of Mediterranean origin. The whole fenugreek plant can be used in herbal medicine, but it is mainly the seeds that are of therapeutic interest. The aim of this study is to strengthen the scientific data on the benefit of using the aqueous extract of fenugreek seeds in the medical field. Our study aims to evaluate the effect of fenugreek on some biochemical parameters of renal function of male rats of the Wistar strain, as well as the study of kidney tissue. The administration of the aqueous extract of fenugreek seeds for 30 successive days (Trigonella foenum-graecum L.) by gavage at a dose of 250g / l of water. In this work, we used 21 male rats, divided into three groups including a control group (T), a group treated with the aqueous extract of fenugreek (F) and a group (G) force-fed with water alone for 30 days by the oral gavage method.Our results show a significant decrease in both blood urea and creatinine levels mainly in group (F) compared to group (T). We therefore found a significant decrease in the serum uric acid (AU) concentration in the fenugreek-treated group (F) compared to the control group (T). The results found reveal that the aqueous extract of fenugreek seeds plays a nephroprotective or even prophylactic role in nephropathologies by improving the parameters of the kidney balance sheet while maintaining their serum levels within the range of normal values, also preservation of intact kidney tissue. Keywords: Fenugreek; Wistar rats; Urea; Creatinine; Uric acid; Kidney.