Micrococcus luteus is now increasingly being recognized as an important human pathogen commonly associated with indwelling device. From a total of 24 urine samples collected from patients with urinary catheter, only 3 M. luteus were identified by using both VITEK 2 automated system and 16S rRNA. In parallel, the organisms evaluated for their in-vitro susceptibility testing and biofilm production. All isolates were found to be susceptible for Chloramphenicol, Ciprofloxacin, Nalidixic acid, Gentamicin, Tetracycline, Novobiocin, Imipenem, Amoxicillin/Clavulanic Acid and Streptomycin antibiotics. Furthermore, we determined the biofilm production by using Microtiter Plate (96-Well Plate) Assay and surfaces (glass, latex and silicon foley sections). We found that all tested isolates show significant capacity to produce biofilm on different surfaces. To the best of our knowledge, we describe for the first time of M. luteus in catheter related urinary tract infection in Iraq.