INTRODUCTION Pseudomonas aeruginosa is a straight or slightly curved, motile gram negative bacilli, strict aerobe that belongs to the family Pseudomonadaceae. It represents a phenomenon of antibiotic resistance and exhibits all known mutational and enzymatic mechanisms of bacterial resistance thus imperilling the selection of appropriate treatment. METHOD Fifty consecutive isolates of Pseudomonas aeruginosa ob tained from various clinical specimens were processed at Central Microbiology Laboratory of Dhiraj Hospital between the periods of December 2023 to May 2024.Specimens were cultured on Blood agar, Mac Conkey agar and Nutrient agar and incubated at 37⁰C for 24 hours. Colonies were identified by its characteristics and biochemical reactions. RESULTS A total of 50 isolates of Pseudomonas aeruginosa was found from clinical samples with 56% samples from pus, 24% from sputum,5% from endotracheal tube secretion, 2% from CSF, and 8% from others. Most of samples were of male 72%. CONCLUSION: Healthcare settings largely contribute as reservoirs of pathogenic strains of Pseudomonas aeruginosa. They are known to utilize their highest levels of intrinsic and acquired resistance mechanisms against most antibiotics.