The leaves of Jatropha gossypifolia Linn. By take on extracts of the leaf prepared with petroleum Ether, methanol, and aqueous medium. Methods: The various experimental models that were used for the evaluation of wound healing, antimicrobial, and anthelmintic activities are as follows: Excision wound model: Wound contraction, epithelization time (days), and area (sq. mm) on complete epithelization were studied. Antimicrobial wound model: Wounds were treated with an antimicrobial agent to decrease the number of microorganisms that caused infection. Anthelmintic worm model Tensile strength (g) measurement of restore incision wound was measured for the incision wound model. Antimicrobial activity was determined make use of the cylindrical plate method on a variety of bacterial and fungal organisms. Pet.ether, methanol, and aqueous leaves extracts of Jatropha gossypifolia Linn. Have been found to display notable wound healing activity as compared to the control. This was insistent using an excision wound model. When differentiate to the control group, all three leaves extracts of Jatropha gossypifolia Linn. Demonstrated a substantial increase in shatter strength in the result incision wound model. It was find that methanol and aqueous extracts had indestructible antibacterial action at concentrations of 25 and 50 mg/ml, despite the fact that pet. Ether extract appear very weak antibacterial activity against gram positive along with gram negative microorganisms. It was discovered that both concentrations of all thrine extracts possessed a level of antifungal activity that ranged from modest to modest when tested on fungal species. Pet. ether, methanol, and aqueous leaf extracts of Jatropha gossypifolia have all demonstrated a low level of action in comparison to the standard medicine.