Objective:To evaluate the impact of age and gender on motor and sensory parameters of nerve conduction studies (NCS), with a focus on the Median nerve and F-wave analysis.Methods:A total of 49 patients underwent NCS to assess the influence of age and gender on nerve function. Key parameters measured included compound motor action potential (CMAP) amplitude, F-wave latency, and sensory nerve action potential (SNAP) amplitude.Results:i) Gender Differences:Males exhibited higher CMAP amplitude and longer F-wave latency. Females demonstrated elevated SNAP amplitude. ii)Age-Related Changes:Increased age was associated with prolonged latencies, diminished amplitudes, and reduced conduction velocities across NCS parameters.Conclusion:The findings highlight the significant influence of age and gender on NCS results. Understanding these variations is crucial for accurate and nuanced interpretation of nerve function assessments, which can enhance clinical decision-making and improve the effectiveness of nerve function evaluations in diverse patient populations.