Anisomeles malabarica is a perennial herb with an extensive history of use in traditional medicine. This summary examines the potential of combining multiple naturally occurring alkali compounds for therapeutic purposes against vector-borne diseases, which have been used to treat illnesses for generations. The current study aims to characterize the antioxidant and antimicrobial properties of alkaloids from AMLE using GC-MS, FTIR, and UV-Vis. AMLE was extracted with the Soxhlet apparatus, and the alkaloid compounds were separated with a separating funnel. Antioxidant properties were investigated with DPPH, ABTS, and H2O2. Antimicrobial properties were tested against a variety of bacterial and fungal species using the agar well diffusion method. Characterization was carried out to determine the presence of bioactive compounds and functional groups. In this study Anisomeles malabarica alkaloids leaf extract exhibited anti-oxidant properties in dose-dependent manner with the IC50 value of 110.2 µg/ml (DPPH), 93.01 µg/ml (ABTS) and 143.4 µg/ml (H2O2). The results revealed maximum anti-bacterial activity and anti-fungal activity against Propionibacterium acnes (15.5±0.7 mm) and Aspergillus niger (8.4±0.56 mm) respectively. GC-MS analysis and FTIR peak values were used to identify the presence of the therapeutic compound chloroxylenol. The functional groups carboxylic acid, aromatic mono-substituted alkene, and aromatic ester were represented. Based on this study, we conclude that alkaloids from AMLE have antioxidant and antimicrobial properties and could serve as a potential target against various diseases.