Volume 6 | Issue -14
Volume 6 | Issue -14
Volume 6 | Issue -14
Volume 6 | Issue -14
Volume 6 | Issue -14
Resveratrol is a major polyphenol with many biological effects, including fighting cardiovascular disease, preventing cancer, and treating diabetes. Extra resveratrol is extracted from grape skins. This study presents a method to detect resveratrol species in grape skins using a high-performance liquid chromatography (HPLC) system.This method is capable of drug selectivity and quantification. The method was validated on a passive Cosmosil C18 analytical column (4.6 X 250 mm, 5 µm) with an equilibration phase consisting of methanol: water (70:30% v/v) at a pH of 3. The flow rate was 0.8 mL/min and the column was maintained at ambient temperature. The photodiode array detector was set at a wavelength of 306 nm. The calibration curve is linear in the concentration range of 10-50 ppm and the correlation coefficient (r2) is 0.999. The interday and intraday precision % RSD values were 0.1480% and 0.09095 %, respectively. The limit of detection and detection were determined to be 0.1329 µg/ml and 0.4028 µg/ml, respectively. This method is validated in terms of precision, accuracy, linearity, limit of detection, quantification of the method. Due to its accuracy, this method can be used for the estimation and analysis of resveratrol in active pharmaceutical products and drugs.