Transcription factors act as the master regulators and control the Cellular processes by decoding the DNA sequence. Plant growth and development is significantly influenced by the DOFs transcription factors. The current study used the DOF genes from Oryza Sativa to conduct a genome-wide investigation of DOF family in Stevia rebaudiana. Different bioinformatics methods were utilized to perform phylogenetic analysis, exon and intro structure, gene structure, chromosomal distribution of various DOFs genes, conserved domain analysis, and subcellular localization prediction. The genome databases 52 DOF genes in Stevia rebaudiana were extracted. Chromosome 4 contained the most SrDOF genes (8 members), followed by chromosome 2 & 11 (7 SrDOF). The majority of SrDOF genes had a one-exon gene structure while mostly DOF genes were intron less. The motif composition and gene structure of SrDOFs from same sub-group were similar. More than 15 conserved motifs were observed in all these proteins. The Zf Dof Superfamily domain was found in all DOF proteins except SrDOF 17 & 20. The molecular weights of 52 SrDOF proteins ranges from 16.7 to 52.16 KDa with average molecular weight 32.6 KDa. Iso electric point of SrDOF proteins range from 4.79 to 9.72 with average PI 7. 89. To conclude, this will help to understand the functions of DOF family in evolutionary and physiological aspects of Stevia rebaudina and provides future direction for novel work to improve crop productivity. Identifying single-gene families in multiple plant species are best to enhance crop productivity, growth, and development.