Pineapple is a versatile fruit and it has different pharmacological properties it is gaining much importancein t h e medicinal field. India is a sub-tropical country and people are suffering from different microbiologicalinfections. As a result, they are taking different synthetic antibiotics from pharmacy shops without knowing theproper course duration and adverse effects. So they are not properly cured and get again infected and becomedrug-resistant. So we are searching for herbal antibiotics that will have good antibacterial activity, fewer sideeffects, and be cost-effective. For this search, we have isolated the different phytoconstituents through column Chromatography and identified t h e m through UV visible spectroscopy and FT-IR. We have investigated the minimum inhibitory concentration and antimicrobial activity. The extract showed different phytochemical andpositive results against different microbes.