In modern times exploiting beneficial microbes as biofertilizers is an intensifying area of research. The excess dosage of synthetic fertilizers has been applied since ages which affected the soil reproductive potential despite increases in yield. The diversity of microbes and the peculiarity of the crops also get scaled-down than usual because the organic content in the soil gets attenuated by repeated application of chemical fertilizers. To reclaim the potential nature of the soil, biological fertilizers or inoculants can be applied, which aids in the reconstruction of the soil's physical structure and microbial growth. In agriculture, germination of seeds is an essential process that determines the crop's potential for growth and productivity. Biofertilizers enrich the soil by converting the unusable form of nutrients to usable form by biological means, these are eco-friendly and cost-effective. In this study isolation of microorganisms from agricultural soils and culturing them in a culture medium by spread plate technique and confirming with biochemical tests, then from the consortium of organisms the less exploited organism was focused. The pigmented actinomycetes were selected for future research in which molecular characterization confirms the species as Rhodococcus pyridinivoran further optimization studies were performed in the outcomes revealed it grows well on pH 7 and temperature 25ºC and in the different combinations of the carbon and nitrogen source C3 & N2 (C3- Fructose & N2- Tryptone + NH4CL) shows better growth. R.pyridinivorans demonstrated notably higher rates of shoot and root elongation and germination in comparison to untreated control plants.