A field experiment was conducted during the kharif season of 2023 to study the molecular, genetic, and biochemical responses of 20 rice genotypes (Oryza Sativa L.) under saline conditions. The experiment, conducted at Genetics & Plant Breeding Farm, Acharya Narendra Deva University of Agriculture and Technology, Kumar Ganj, Ayodhya (U.P.), utilized a Randomized Block Design. Analysis of variance indicated significant variability among genotypes for eleven traits. Phenotypic coefficient of variation (PCV) was highest for grain yield, harvest index, spikelet number, biological yield per plant, and grains per panicle. High heritability estimates were observed for grain yield, spikelet number, biological yield per plant, grains per panicle, and harvest index. Medium heritability estimates were noted for panicle bearing tillers per plant, test weight, spikelet fertility, plant height, panicle length, and days to 50% flowering.