Background: Shampoos are most likely used for cosmetic purposes. It is a product for hair care and we use it in daily life to clean our hair and scalp. Shampoos are viscid preparations of detergents with appropriate additives, protective substances, and active compounds that are most commonly used as beautifying agents. Objective: This study aims to prepare and assess polyherbal shampoos with or without SLS for cosmetic purposes from herbal ingredients. Methodology: The two polyherbal shampoos were formulated in which one was prepared by using SLS and another one was prepared without SLS. The preparation of polyherbal shampoo has two sections, the first section contains, a polyherbal extract prepared by a mixture of Reetha, Amla, Kala siris, Neem, Jatamansi, Bhringraj, Linseed, Aloe vera, and some ingredients. Herb extract of all herbs was added to pestle mortal and mixed properly with a specified quantity of water (A). In the second section, the mortar contains glycerin, propyl glycol, and with or without SLS was stirred gradually (B). Now sample B is mix by adding it to sample A. Preservative methylparaben was added into it. Pour Aloe vera juice into it and thickness was increased by adding tragacanth gum and making up volume with water. For quality assurance purposes, the developed polyherbal shampoos were also compared with the commercially available shampoos.