Type 2 diabetes mellitus (T2DM) is a chronic metabolic disorder characterized by hyperglycemia due to insulin resistance and/or impaired insulin secretion. This study aimed to evaluate the association between various biochemical markers and glycemic control in T2DM patients. A cross-sectional analysis was conducted on 200 patients recruited from a tertiary care hospital. Data collection included demographic information, anthropometric measurements, and laboratory tests for fasting plasma glucose (FPG), glycated hemoglobin (HbA1c), lipid profile, adipokines (adiponectin, leptin), inflammatory markers (CRP, IL-6), and oxidative stress markers (MDA, TAC). Results indicated significant correlations between several biochemical markers and HbA1c levels. FPG, total cholesterol, LDL-C, CRP, IL-6, and MDA were positively correlated with HbA1c, indicating poorer glycemic control. Conversely, adiponectin and TAC showed negative correlations with HbA1c, suggesting a protective role. Multiple linear regression analysis identified FPG, CRP, and adiponectin as significant independent predictors of HbA1c levels. The findings underscore the critical role of a comprehensive panel of biochemical markers in the management of T2DM. Incorporating inflammatory and oxidative stress markers into routine monitoring could enhance the precision of glycemic control strategies and improve patient outcomes. Future research should focus on longitudinal studies and intervention trials to further elucidate these associations and develop targeted therapeutic interventions.